The process of cultivating the mother strain of Tremella involves isolating and purifying both the white fungus mycelium and the incense mycelium from test tube cultures. When producing the mother strain, the white fungus mycelium is first removed and expanded to the required number of tubes. It is then cultured at 25°C until it reaches approximately 1 cm in diameter. At this stage, a small amount of ash mycelium is inoculated into each test tube containing long mycelium growth. Once combined, the two types of mycelium can be sold or used for further production of the original species.
If the ash mycelium is introduced first, it grows rapidly, covering the surface and consuming nutrients quickly. This makes it difficult for the white fungus mycelium attached to it to grow effectively. Similarly, if both the white fungus and tremella mycelia are inoculated simultaneously into a test tube, there may be an imbalance, with the white fungus mycelium being insufficient and the other type overgrowing.
Therefore, when producing the original species of Tremella (test tube strains), the order of inoculation and the proportion between the two types of mycelium must be carefully controlled.
In addition to the white fungus mycelium, the formation of fruiting bodies in Tremella also requires the presence of "associated bacteria," specifically the ash gray bacteria. Similarly, the golden ear fungus requires the help of hairy hard leather fungi. Under normal conditions, pure mycelium of Tremella fuciformis and Auricula grows slowly and has weak ability to decompose lignin and cellulose. They rely on the decomposition of the substrate by the hyphae and the coarse hairs and harder hyphae to provide nutrients. Without this support, fruiting bodies cannot form. Thus, during propagation, the two corresponding mycelia must be cultured together.
One method of mixed culture is the inclined hybrid parent technique. This involves placing PDA slant tubes with Tremella mycelium and new test tubes in the inoculation room. Using aseptic techniques, a small piece of culture medium is transferred onto the slant surface of a blank test tube. After incubation at 22-24°C for 6-8 days, the white fungus mycelium grows to about the size of a soybean. It is then transferred along with the ash mycelium into the inoculation chamber, where a small piece of the mycelium is inserted approximately 2 cm away from the white fungus mycelium. After 2 days, white mycelium appears on the block. By day 7, a white hairball forms around the white fungus mycelium, and by day 12-15, red and yellow beads appear, indicating success.
It is important to note that when using mixed parents of Tremella and Coprinus comatus to expand the original species, only the white fungus mycelium resembling hydrangea and the black ash mycelium near it should be used. Therefore, one bottle of original species can be connected to one slant mother.
Another method is the bottled sawdust original mixed method. If pure Tremella mycelium is not available, spores can be used to cultivate the spore mother, which is then transferred to a wooden culture medium made from Coprinus species. The ratio of 1:15 incense ash slant mother to 12-15 bottles of sawdust medium is typically used. After aseptic inoculation, the Coprinus mycelium is inserted into the sawdust medium. After 2 days, white hyphae begin to appear. By day 3, bacterial contamination is checked and removed. After 11 days, the hyphae extend about 3 cm into the medium. Dark secretions on the surface indicate vigorous growth, and spore mothers should be inserted promptly.
Using a 1:12-15 ratio, the Tremella spore mother test tube and the wood culture medium from Coprinus comatus are placed in the inoculation box. A sterile knife cuts the incense mycelium, and a small amount of Tremella spores is picked up with a sterile spatula and inserted into the incense mycelium. After mixing, the bottles are incubated at 23-25°C. By day 10, thick white hyphae appear. After 18 days, red and yellow droplets emerge, and by day 25, twisted fruiting bodies are observed, indicating successful cultivation. One bottle of this mixture can be expanded to 30-40 bottles of original species.
The production of planting species depends on the quality of the original species, the size of the ear base, and the depth of the white fungus mycelium's colonization. To ensure quality, it is recommended to expand within 40-60 bottles per original bottle. If the original species is well-mixed, it can potentially expand to 100-200 bottles.
To produce high-quality Tremella species, strict aseptic procedures must be followed. Additionally, the combination of white fungus mycelium and ash hyphae must be specific. Only pure cultures from the same ear should be used. Mixing mycelium from different ears or regions can lead to poor results. Any mycelium that successfully colonizes the medium, forms an ear base, and opens properly can be used for further production.
The inoculation ratio is crucial. Since the ash mycelium grows much faster than the white fungus, the white fungus must be inoculated first. Ideally, the ratio should be 1000:1. Otherwise, the white fungus may not grow sufficiently, leading to low ear rates. Adding yeast-like conidia to the coniferous mycelium can increase conidial numbers, but not all will germinate. Thus, this method may not yield optimal results. Instead, concentrating the conidia on the surface of the ash mycelium improves germination.
Cultivation conditions vary between pure and mixed cultures. Temperature should be maintained between 20-28°C, preferably 23-25°C. Moisture content for the original species should be around 65%, while planting species require 65-70%. A slightly drier medium promotes white fungus growth without excessive conidia formation, making it easier to expand into planting species. A slightly wetter medium supports conidia development and better nutrient distribution.
Before expanding the original species into planting species, check the fertility of each bottle. Bottles with large ear groups, deep white fungus mycelium, and strong ash mycelium can be expanded more. Otherwise, they should not be used. To ensure quality, it is advisable to expand 40-60 bottles per original bottle. After mixing, the cotton plug should be reinserted and the culture continued for 3-5 days to regenerate the white fungus mycelium.
Long-term use of strains can lead to decreased viability, reduced ear formation, and poor regeneration. Different strains should be selected based on the cultivation method. For example, Duanmu planting requires young, low-maturity strains with strong aerial mycelium, while bottle planting needs mature strains with smaller, gelatinized mycelium. The incense ash mycelium should be slightly weakened in some cases to avoid consuming all nutrients.
Original species stored too long may have reduced viability and should be discarded. Freshly inoculated media must contain both white fungus mycelium and ash mycelium, or yeast-like conidia and ash mycelium. If not used immediately, the original species should be stored in a cool, dry place. Planting should occur within 45 days.
Pure Tremella strains (mycelium and spores) should be preserved separately. Long-term storage can cause yellowing, while mixed cultures are less prone to discoloration. To maintain color stability, it is recommended to select wild or domestic strains that retain their white color for over three years.
As age increases, the skin gradually loses elasticity, wrinkles, and becomes loose and dull. Anti-aging aims to help slow down the process of the skin aging.
Sustained Release Retinol, Food Additive Vitamin A Acetate,itamin A Acetate Benefits
Xi'an Hersoncare Bio-Tech Co., Ltd , https://www.hersoncarebio.com