The process of producing the mother strain of Tremella involves isolating and purifying the white fungus mycelium and the ash mycelium from a test tube culture. When preparing the mother strains, the white fungus mycelium is first removed and expanded to the required number of tubes. It is then cultured at 25°C until the Tremella reaches approximately 1 cm in diameter. At this stage, a small amount of ash mycelium is inoculated into each test tube containing the long-growing mycelium. This combination can be sold or used for further production of the original species.
If the ash mycelium is introduced first, it grows rapidly, covering the medium and consuming nutrients quickly. As a result, the attached white fungus mycelium struggles to grow effectively. Similarly, if both the white fungus and ash mycelia are inoculated simultaneously, the white fungus may not develop properly due to an imbalance in growth rates.
Therefore, when producing the original species of Tremella (test tube species), the order of inoculation and the ratio between the two types of mycelium must be carefully controlled.
In addition to the pure white fungus mycelium, Tremella requires the presence of "associated bacteria," specifically the ash mycelium. These bacteria help decompose the substrate, providing essential nutrients for the growth of Tremella. Without this symbiotic relationship, the fruiting bodies cannot form. The same applies to the golden ear fungus, which also needs specific associated fungi for successful development.
Under normal conditions, the pure mycelium of Tremella fuciformis and Auricula grows slowly and has weak lignin and cellulose-degrading abilities. They rely on the assistance of the associated hyphae to break down the substrate and supply necessary nutrients. Therefore, during propagation, it's crucial to mix the two types of mycelia on the same medium.
One method for mixed cultivation is the inclined hybrid parent method. In this technique, a test tube containing Tremella mycelium is placed alongside a new PDA slant tube in the inoculation room. After aseptic transfer, a small piece of the culture medium is used to inoculate the slant. Once the white fungus mycelium grows to a size similar to a soybean, it is transferred along with the ash mycelium. After a few days, the white hyphae begin to grow around the initial culture, eventually forming a white ball that covers the pure mycelium. Red and yellow beads appear after about 12-15 days, indicating success.
Another method is the bottled sawdust original mixed method. If pure Tremella mycelium is unavailable, spores can be collected and used to inoculate a wood-based medium. A proper ratio of ash mycelium to sawdust medium is maintained, and after several days of incubation, the hyphae spread through the medium, signaling successful growth.
The number of bottles expanded from one original species depends on the quality of the original strain, the size of the ear base, and how deeply the white fungus mycelium has penetrated the medium. To ensure quality, it is recommended to expand within 40-60 bottles per original. However, if the mixture is evenly stirred, up to 100-200 bottles may be possible.
To produce high-quality Tremella, strict aseptic techniques must be followed. Additionally, the specific combination of white fungus mycelium and ash hyphae must be from the same source. Mixing mycelia from different ears or regions may lead to poor results. Only those strains that continue to grow, form an ear base, and open smoothly should be used for production.
The inoculation ratio is also critical. Since the ash mycelium grows much faster than the white fungus, it is best to first cultivate the white fungus before introducing the ash. A ratio of 1000:1 for white fungus to ash is ideal. Otherwise, the white fungus may be outcompeted, leading to low ear formation.
Cultivation conditions vary between pure and mixed cultures. Temperature should be kept between 20-28°C, with 23-25°C being optimal. Moisture levels should be controlled at 65% for the original species and 65-70% for planting species. Proper moisture ensures better conidia germination and even distribution of hyphae throughout the medium.
Before expanding the original species into planting species, each bottle must be checked for fertility. Those with strong, deep white fungus mycelium and thick ash mycelium are ideal for expansion. If the original species is too old or shows signs of decline, it should be discarded to maintain quality.
Finally, the original species should be stored in a refrigerator at 4-15°C and re-inoculated every 1-3 months. If not used immediately, it should be kept in a cool, dry place. Planting should occur within 45 days to ensure viability. Long-term storage of pure strains may lead to discoloration, but mixed cultures tend to remain stable over time. For best results, strains should be selected from healthy, young cultures to preserve their color and vitality.
The colorant provided by our company are all natural colorant. Natural colorant are edible colorant obtained from natural resources. It is mainly extracted from plant tissues and fermented by microorganisms.
Most plant colorant have no side effects and are highly safe. Plant colorant are mostly anthocyanins, carotenoids, and flavonoid compound, which are a kind of bioactive substances and functional active ingredients in plant medicine and health food.
In the application of health food, this type of plant pigment can play auxiliary roles such as enhancing human immune function, antioxidation, and reducing blood fat. In ordinary food, some can play the auxiliary role of nutritional enhancement and antioxidation.
Organic Chlorella Powder,Marigold Flower Extract,Chlorella Protein Powder,Organic Certified Blue Spirulina Powder
Xi'an Hersoncare Bio-Tech Co., Ltd , https://www.hersoncarebio.com