Product configuration : air pump, anesthesia machine, isoflurane, induction box, anesthesia mask
Anesthesia machine and accessories connection diagram
Steps:
1. Carefully check that the piping connections are correct and that the piping is intact and airtight;
2. Unscrew the filling sealing cap at the front end of the evaporator of the anesthesia machine, slowly pour the anesthetic along the central sealing screw (see the diagram attached to the evaporation canister during the pouring process), and observe the liquid level marking on the front of the evaporator at any time. Make sure that the anesthetic liquid level is between the upper and lower scale lines. After the anesthetic is installed, lock and seal the sealing cap;
3. Switch the three-way valve switch to ensure that the airflow from the anesthesia evaporator exits the anesthesia induction box (at this point, the switch should point in the direction leading to the anesthesia mask);
4. Check if the anesthesia machine evaporator is off (rotate the dial so that the “OFF†or “O†position is aligned with the “∣†mark on the front of the evaporator);
5. Connect the air pump power supply, and turn on the air pump switch to rotate and adjust the air source valve at the front end of the oxygen flow meter to make the output gas reach the required flow rate (typically adjusted to 500-700ml/min for rats, generally for mice) Adjusted to 300-500ml/min), the required gas flow rate is mainly determined by the animal's species, weight and animal status;
6. Open the evaporator: while pressing the control button, turn the dial counterclockwise to align the concentration value of the required anesthetic with the “∣†mark on the front of the evaporator, and release the control button. The corresponding value is anesthesia. The percentage concentration of gas in the mixed gas;
7. After the induced concentration is adjusted (the general induced concentration is adjusted to 3-4%), the anesthetic is filled with the induction box. After about 1 minute, the animal is placed in the induction box, and then the induction box is closed, waiting for the animal to be completely anesthetized (this process is required) 2-3 minutes). The animal can be completely anesthetized by gently shaking the induction box. If the animal body is turned upside down and does not attempt to return to its lying position, the animal is completely anesthetized;
8. Convert the three-way valve switch to ensure that the airflow from the anesthesia machine evaporator is connected to the anesthesia mask (the switch should point in the direction of the induction box);
9. After maintaining the concentration adjustment (mainly maintained at a concentration of 2-2.5% for rats; generally maintain a concentration of 1-1.5% for mice), remove the animal from the induction box and place the head/nose in an anesthesia mask. And check whether the animal is in full anesthesia (the animal's paws or tail can be pinched with two fingers. If the animal does not respond, it indicates that the animal has been completely anesthetized, and surgery can be started at this time);
10. After the animal experiment is completed, close the evaporator (operation is the same as step 4), keep the animal breathing in pure oxygen for about 5-10 minutes, in order to facilitate the animal to wake up quickly.
Special note : In order to eliminate the cause, the original anesthetic in the anesthesia machine evaporator can be drained to test our isoflurane effect. The steps are as follows:
1. Unscrew the sealing cap to expose the drain sealing screw, reverse the sealing cap and use the groove at the top of the sealing cap to loosen the sealing screw, and put the discharged medicament into a labeled suitable container for subsequent processing; Tightly seal the screw; restore the sealing cap.
2. Fully open the anesthesia machine evaporator (5%), pass pure oxygen or air (gas flow can be between 500-1000ml/min), maintain for about 10 minutes, completely empty the residual anesthetic of the evaporator, then Then press "Operation Steps" to continue using.
Scented tea is also known as fruit grain of tea, is a kind of tea drink, in a variety of flowers and fruit drying, concentrate composition contains a variety of vitamins, fruit acid and minerals, but contains no caffeine and tannic acid, a variety of different flavors of scented tea is slightly different in composition, after brewing can still maintain the original flavor of fruit, rich aroma, add rock sugar drink, It can relieve mood and have the effect of beauty.
Flower and fruit tea, half is used to taste, half is used to appreciate, can not be put in a crock. When making tea, it is best to choose a transparent glass pot. It is also a kind of enjoyment to see the glittering and translucent tea water a little bit by the petals. In the brewing process of flower and fruit tea, the temperature should be slightly higher than that of general tea. Because the general tea has been rolled in the process of processing this process, and the flower and fruit tea in the production is generally dry after drying.
It should be noted that on the premise of brewing the flavor of tea soup, the brewing temperature should not be as high as possible, because some effective active substances in tea, such as polyphenols and flavonoids, will decompose at high temperature, so that the efficacy is damaged. Generally speaking, the higher the brewing temperature, the more bitter taste in the tea, can be appropriate to add a little orange juice, honey and other bitter. Of course, if you like a strong taste, you can also cook it.
Storage: The general storage method is to use a dry container, stored in a cool, dry environment. Drink as soon as possible after opening to ensure freshness. To serve, use a dry spoon. It is recommended to put scented tea into the refrigerator freezer, pay attention to storage should be away from fishy smell, avoid fish, seafood and other food put together.
Fruit Tea,Hawthorn Berry Tea,Raspberry Tea,Mango Bubble Tea
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