Shiitake cultivation technology

Main technical contents: 1. Variety selection: Choose high-quality, high-yield, and strong stress-resistant varieties suitable for northern climate characteristics, especially high-temperature resistant varieties such as Xiang 66, Wuxiang No. 1, L26, Cr04, L937, L867 and so on. 2, the cultivation season: sowing time from late March to late April, the surface defrosting more than 10cm began to sow. When the temperature rose to 8 °C ~ 10 °C, the end of sowing. 3, the selection of land, do 畦 畦: choose a well-ventilated, non-flood and drought, not heavy soil and sandy soil, away from the source of pollution. Trampoline east-west, can be south or north. Plots or sloping areas with higher ground should be low-lying; low-lying plots should be flat or squat. 4, training materials: The main raw material is best to use hard mixed wood chips, can not have mildew or mixed organic oil, the thickness of the other half; add a certain amount of bran in the culture material, rice bran, cornmeal, soybean meal and other fresh accessories. 5, sowing: sowing rate of 12% to 15% of dry material, using mixed broadcast plus seeding method. Horizontally ventilate the straw and place it on the straw and one on the film. Then fold back the film on the other side to wrap the material and expose the grass to the outside of the film. 6, cover the grass cover: cover the soil after the film, avoid covering fine soil. The straw shall be exposed on the surface of the cover soil with a thickness of 3cm to 5cm. When the maximum temperature exceeds 25°C, the prepared straw curtain shall cover the surface of the bed so as not to expose light. 7. Fungus management: 30 days to 40 days during germination period, 15 days after sowing, strengthen management, prevent bacteria from condensing, and timely ventilation. 8, remove the soil uncovering film, support arch curtain: after sowing 30d ~ 40d shake off the residual film on the film, uncover the plastic film uncover film, cuttings, arch arched arched edge, side straw, two grass curtain Between 3cm and 5cm should be overlapped. 9, color management: 7d after the film was ventilated. Select the windless days, after 10:00 am or after 3 pm, remove the membranes for the second ventilation after the second week, the time is 0.5 h. Scattered light on the bed can meet the light requirements of the mycelium turning color to achieve normal color conversion. If straw curtains are too thick, straw curtains can be opened in the morning and evening for light. 10, mushroom management: 60d ~ 70d after sowing, on both sides of the trampoline reported mushrooms. From early July to early August, when the temperature reaches 25°C or more, over-summer management is conducted. After the beginning of autumn, when the minimum temperature dropped to 20°C, it began to fall. 11. Pest control: For diseases such as Trichoderma, Penicillium, Green mold, Aspergillus, etc., measures such as spraying lime or ventilation, drainage, scraping, and deep burial shall be adopted; pests such as fly, mosquitoes and other insects may be captured manually or In the small shed hangs the method such as the poisonous killing of the flumethrin cotton wool. 12. Harvesting: When the fruiting body grows to seven or eight minutes, it should be picked in time. For the preservation of mushrooms, harvesting is done when the mushroom screen is not cracked.

Sorfa Cell Culture Plates (also named tissue culture plates) are manufactured from raw materials of high transparency polystyrene in four different sizes: 6 wells, 12 wells, 24 wells and 96 wells. Flat surface of bottom in uniformity maximize growth area for each well. Special design of chamfers and raised rims on wells mated rings on inner surface of lid lessen cross contamination among wells. Stacking ring on the lid stabilize plates when stacking multi layers for saving space in laboratory.


Compared with traditional plate, special design of anti-skid area on side face is easy for grip. The arrays of numerical code greatly help to identify each well for various medium and purposes. For TC surface treatment, plates are available for suspension culture and adherent culture to meet global market need. All Sorfa cell culture plates are packaged with sterilization, RNase-free, DNase-free and Non-pyrogenic.


Product Feature:

1. High transparency polystyrene.

2. Anti-slip area on base sides is easy to grip.

3. Numerical codes are for easy identification.

4. Sterilized by Gamma irradiation.

5. TC treated and Non-treated are both available.

6. RNase-free, DNase-free and Non-pyrogenic.


Packaging information:

Lot number is printed on packaging for tracking

Plastic/paper packaging is easy to open

Cartons are customized for protecting products.





Cell Culture Plates

Cell Culture Plates, Plastic Cell Culture Plate, Disposable Cell Culture Plate

Zhejiang Sorfa Medical Plastic Co., Ltd. (Ningbo Jiangdong Aron Imp&Exp. Co.LTD) , http://www.sorfapipettes.com

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