The cultivation of *Coprinus comatus*, commonly known as shiitake or "chicken leg mushroom" due to its shape resembling chicken legs, is a valuable and rare edible fungus that has gained commercial interest in recent years. It's often referred to as the "new player in the bacterial world" because of its unique growth characteristics. The production of its cultivation materials requires strict temperature control throughout the fermentation process.
During composting, microorganisms in the starter culture play a crucial role by breaking down organic materials such as straw or other fibrous substrates. As these microbes grow and multiply, they generate heat through biochemical reactions, which helps break down the plant tissues, remove wax from the surface, and release nutrients beneficial for *Coprinus comatus* growth. This process also improves the physical structure of the material, making it more porous and easier to absorb moisture. Additionally, it ensures even mixing of components and balanced nutrition.
Cellulose breakdown in the cultivation medium mainly depends on microbial activity. The compost piles must be carefully managed—too high or too wide, and the temperature may rise beyond the optimal range; too low or too narrow, and the pile won't retain enough heat. The ideal temperature for cellulose decomposition by most microorganisms is between 50–65°C. When the pile reaches above 70°C, fiber-decomposing bacteria begin to slow down, and at over 80°C, many microorganisms are killed off. At such high temperatures, some nutrients can be lost, and water evaporates rapidly.
However, if the pile is too small, it may not hold enough moisture or heat, which hinders microbial activity. Therefore, during the production of *Coprinus comatus* cultivation materials using microbial fermentation agents, it's essential to maintain proper temperature levels and regularly turn the material to ensure even decomposition and nutrient availability.
Fermentability microbe, a type of microbial agent used in this process, contains a variety of microorganisms including spores, yeasts, and lactic acid bacteria. It features multiple temperature zones (from 0°C to 70°C), allowing it to adapt to different seasonal conditions and support efficient composting throughout the year.
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